Modulation of Duodenal TfR Expression in IDA Induced Rat with Date Palm and Goat Milk Intake
DOI:
https://doi.org/10.56532/mjsat.v4i1.207Keywords:
Date palm, Goat milk, Iron deficiency anemia, Transferrin receptorAbstract
Iron deficiency anemia (IDA) is a medical condition characterized by insufficient iron levels in the body, resulting in a reduced ability to produce hemoglobin (Hb), a critical component of red blood cells. IDA is often associated with chronic fatigue, impaired cognitive function, and diminished well-being. Transferrin (Tf) is one of the major proteins in iron homeostasis, responsible for transporting iron through the blood to various tissues, while its carrier protein, transferrin receptor (TfR), mediates the cellular uptake of transferrin-bound iron into the cell. This study aims to evaluate the expression of TfR mRNA and protein in the small intestine following intervention with date palm and goat milk in IDA-induced rats. Twenty-four male Wistar rats were induced with IDA for 2 weeks using a low-iron diet. Following IDA detection, rats were supplemented with date palm and goat milk, singly and in combination. After four weeks, the rats were sacrificed, and the expression of TfR mRNA and protein in the small intestine was assessed using qPCR and immunohistochemistry, respectively. Data were analyzed using SPSS 24.0, with a significance level set at p<0.05. Results demonstrated that date palm and goat milk significantly improved Hb, serum iron, Tf saturation levels, and modulated the expression of TfR mRNA in the IDA-induced rats. Expression of TfR on the crypt region and brush border membrane of the small intestine was normalized following intervention. The findings indicate that supplementation of date palm and goat milk improved Hb and Tf saturation levels and significantly modulated duodenal TfR expression in IDA-induced rats.
References
NHMS, “National Health and Mobidity Survey 2019. Non-communicable disease, healthcare demand, and health literacy; Key Findings,” 2019.
WHO, “Anaemia in Women and Children: WHO Global Anaemia Estimates, 2021 Edition.,” World Health Organization, 2021. [Online]. Available: https://www.who.int/data/gho/data/themes/topics/anaemia_in_women_and_children. [Accessed: 02-Sep-2022].
S. Safiri et al., “Burden of anemia and its underlying causes in 204 countries and territories, 1990–2019: results from the Global Burden of Disease Study 2019,” J. Hematol. Oncol., vol. 14, no. 1, pp. 1–16, 2021.
M. D. Cappellini, K. M. Musallam, and A. T. Taher, “Iron deficiency anaemia revisited,” J. Intern. Med., vol. 287, no. 2, pp. 153–170, 2020.
D. Galaris, A. Barbouti, and K. Pantopoulos, “Iron homeostasis and oxidative stress: An intimate relationship,” Biochim. Biophys. Acta - Mol. Cell Res., vol. 1866, no. 12, p. 118535, 2019.
A. C. S. Vogt, T. Arsiwala, M. Mohsen, M. Vogel, V. Manolova, and M. F. Bachmann, “On iron metabolism and its regulation,” Int. J. Mol. Sci., vol. 22, no. 9, pp. 1–17, 2021.
E. Gammella, P. Buratti, G. Cairo, and S. Recalcati, “The transferrin receptor: The cellular iron gate,” Metallomics, vol. 9, no. 10, pp. 1367–1375, 2017.
A. C. Chen, A. Donovan, R. Ned-Sykes, and N. C. Andrews, “Noncanonical role of transferrin receptor 1 is essential for intestinal homeostasis,” Proc. Natl. Acad. Sci. U. S. A., vol. 112, no. 37, pp. 11714–11719, 2015.
S. Recalcati, E. Gammella, P. Buratti, and G. Cairo, “Molecular Regulation of Cellular Iron Balance,” IUBMB Life, vol. 69, no. 6, pp. 389–398, 2017.
Division of Family Health Development, “Perinatal Care Manual 4th Edition,” 2020.
R. Abd Rahman, I. B. Idris, Z. M. Isa, R. A. Rahman, and Z. A. Mahdy, “The Prevalence and Risk Factors of Iron Deficiency Anemia Among Pregnant Women in Malaysia: A Systematic Review,” Front. Nutr., vol. 9, no. April, pp. 1–9, 2022.
N. A. Kadir, N. A. A. Rahim, E. Mangantig, N. A. Z. N. Lah, and A. H. Ahmad, “Knowledge of oral iron consumption among pregnant women at Hospital Universiti Sains Malaysia,” Malaysian J. Med. Heal. Sci., vol. 17, no. 9, pp. 107–117, 2021.
N. A. A. Daud, S. A. Mukhlef, and F. A. Aziz, “Awareness, attitude and behaviour related to the intake of dietary supplements among Malaysian pregnant women,” Malaysian J. Med. Heal. Sci., vol. 16, no. 4, pp. 81–88, 2020.
S. García López, A. Rubio, V. Poza, Bermejo, and S. García López, “Optimal management of iron deficiency anemia due to poor dietary intake,” Int. J. Gen. Med., vol. 1, no. 4, pp. 741–750, 2011.
J. P. Chouraqui, “Dietary Approaches to Iron Deficiency Prevention in Childhood—A Critical Public Health Issue,” Nutrients, vol. 14, no. 8, pp. 1–24, 2022.
A. Heffernan, C. Evans, M. Holmes, and J. B. Moore, “The Regulation of Dietary Iron Bioavailability by Vitamin C: A Systematic Review and Meta-Analysis,” Proc. Nutr. Soc., vol. 76, no. OCE4, p. 4433, 2017.
N. A. Majid, Z. Zainol, N. A. Shamaan, N. A. Hamid, N. Roslan, and N. F. Zulkifli, “Date palm and goat milk improve haematological parameters and availability of functional iron in iron deficient rats,” Malaysian J. Med. Heal. Sci., vol. 16, no. 3, pp. 52–59, 2020.
M. M. Abdelsalam, M. M. Zeitoun, M. A. Ateah, A. Al-Hassan, and A. M. Abdel-Salam, “Impact of probiotic fermented milk, palm date extract and their mixture supplementation on neonatal traits and hematological parameters of late pregnant najdi ewes,” International Journal of Biological Chemistry, vol. 8, no. 1. pp. 37–47, 2014.
A. T. H. Zen, D. Pertiwi, and Chodidjah, “Pengaruh Pemberian Sari Kurma (Phoenix dactylifera) terhadap Kadar Hemoglobin Studi Eksperimental pada Tikus Putih Jantan Galur Wistar yang Diberi Diet Rendah Zat Besi (Fe),” Sains Med., vol. 5, no. 1, pp. 17–19, 2013.
S. N. Onuh, E. O. Ukaejiofo, P. . Achukwu, S. A. Ufelle, C. N. Okwuosa, and C. J. Chukwuka, “Haemopoietic activity and effect of Crude Fruit Extract of Phoenix dactylifera on Peripheral Blood Parameters,” Int. J. Biol. Med. Res., vol. 3, no. 1, pp. 1720–1723, 2012.
J. Díaz-Castro, M. J. M. Alférez, I. López-Aliaga, T. Nestares, and M. S. Campos, “Effect of calcium-fortified milk-rich diets (either goat’s or cow’s milk) on copper bioavailability in iron-deficient anemia,” Dairy Sci. Technol., vol. 91, no. 2, pp. 203–212, 2011.
M. J. M. Alférez et al., “Dietary goat milk improves iron bioavailability in rats with induced ferropenic anaemia in comparison with cow milk,” Int. Dairy J., vol. 16, no. 7, pp. 813–821, 2006.
J. Diaz-Castro, M. J. M. Alférez, J. Moreno-Fernandez, and I. López-Aliaga, Influence of Goat Milk on Bone and Mineral Metabolism During Iron Deficiency Recovery. Elsevier Inc., 2017.
Y. Q. Li, B. Bai, Q. Q. Zheng, H. Yan, and G. H. Zhuang, “Quantitative Study of Iron Metabolism-related Genes Expression in Rat,” Biomed. Environ. Sci., vol. 26, no. 10, pp. 808–819, 2013.
F. Dupic et al., “Duodenal mRNA expression of iron related genes in response to iron loading and iron deficiency in four strains of mice,” Gut, vol. 51, pp. 648–653, 2002.
A. Mckie, K. Raja, T. Peters, F. Farzaneh, and R. Simpson, “Expression metabolism of genes involved in iron in mouse intestine,” Am. J. Physiol., vol. 271, pp. G772–G779, 1996.
S. Deaglio et al., “Structural , functional , and tissue distribution analysis of human transferrin receptor-2 by murine monoclonal antibodies and a polyclonal antiserum,” Blood, vol. 100, no. 10, pp. 3782–3789, 2002.
V. L. Kolachala, B. Sesikeran, and K. M. Nair, “Evidence for a sequential transfer or iron amongst ferritin, transferrin and transferrin receptor during duodenal absorption of iron in rat and human,” World J. Gastroenterol., vol. 13, no. 7, pp. 1042–1052, 2007.
A. Darwich, U. Aslam, D. Ashcroft, and A. Rostami-Hodjegan, “Meta-analysis of the turnover of intestinal epithelia in preclinical animal species and humans,” Drug Metab. Dispos., vol. 42, no. 12, pp. 2016–2022, 2014.
S. H. Yoon, D. S. Kim, S. T. Yu, S. R. Shin, and D. Y. Choi, “The usefulness of soluble transferrin receptor in the diagnosis and treatment of iron deficiency anemia in children,” Korean J. Pediatr., vol. 58, no. 1, pp. 15–19, 2015.
A. T. Idowu, O. O. Igiehon, A. E. Adekoya, and S. Idowu, “Dates palm fruits: A review of their nutritional components, bioactivities and functional food applications,” AIMS Agric. Food, vol. 5, no. 4, pp. 734–755, 2020.
D. J. R. Lane and D. R. Richardson, “The active role of vitamin C in mammalian iron metabolism: Much more than just enhanced iron absorption!,” Free Radic. Biol. Med., vol. 75, pp. 69–83, 2014.
M. B. Mirza, F. Q. Syed, F. Khan, A. I. Elkady, A. M. Al-Attar, and K. R. Hakeem, “Ajwa dates: A highly nutritive fruit with the impending therapeutic application,” Plant Hum. Heal. Pharmacol. Ther. Uses, vol. 3, pp. 209–230, 2019.
M. J. R. Lima, E. Teixeira-Lemos, J. Oliveira, L. P. Teixeira-Lemos, A. M. C. Monteiro, and J. M. Costa, “Nutritional and Health Profile of Goat Products: Focus on Health Benefits of Goat Milk,” in Goat Science, S. Kukovics, Ed. Portugal: Licensee IntechOpen, 2018, pp. 189–232.
N. Turkmen, “The Nutritional Value and Health Benefits of Goat Milk Components,” in Nutrients in Dairy and Their Implications for Health and Disease, W. Ronald, R. Collier, and V. Preedy, Eds. Elsevier Inc., 2017, pp. 441–449.
M. S. Campos, I. López-Aliaga, M. J. M. Alférez, T. Nestares, and M. Barrionuevo, “Effects of goats’ or cows’ milks on nutritive utilization of calcium and phosphorus in rats with intestinal resection,” Br. J. Nutr., vol. 90, no. 1, pp. 61–67, 2003.
M. Barrionuevo, M. J. M. Alferez, I. Lopez Aliaga, M. R. Sanz Sampelayo, and M. S. Campos, “Beneficial Effect of Goat Milk on Nutritive Utilization of Iron and Copper in Malabsorption Syndrome,” J. Dairy Sci., vol. 85, no. 3, pp. 657–664, 2002.
M. Barrionuevo, I. López Aliaga, M. J. M. Alférez, E. Mesa, T. Nestáres, and M. Sánchez Campos, “Beneficial effect of goat milk on bioavailability of cooper, zinc and selenium in rats,” J. Physiol. Biochem., vol. 59, no. 2, pp. 111–118, 2003.
I. López-Aliaga, J. Díaz-Castro, T. Nestares, M. J. M. Alférez, and M. S. Campos, “Calcium-supplemented goat milk does not interfere with iron absorption in rats with anaemia induced by dietary iron depletion,” Food Chem., vol. 113, no. 3, pp. 839–841, 2009.
J. Díaz-Castro, M. J. M. Alférez, I. López-Aliaga, T. Nestares, and M. S. Campos, “Effect of calcium-fortified milk-rich diets (either goat’s or cow’s milk) on copper bioavailability in iron-deficient anemia,” Dairy Sci. Technol., vol. 91, no. 2, pp. 203–212, 2011.
Y. W. Park, “Nutritional and Chemical Composition of Goat Milk,” Handb. Milk Non‐Bovine Mammals, Second Ed., no. 119, pp. 34–58, 2017.
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